| Screen a library of point mutants of your favorite protein |
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and identify the critical residues required for binding to its partners.A single amino acid can make the difference.Our Loss of Affinity Mutants (interaction-deficient) assay is based on Hybrigenics Services’ ULTImate Y2H™ . The mutagenic conditions of the library are adapted to your protein in order to obtain a single mutation per clone on average. The sequences of 288 clones showing no or weak interaction are aligned and interaction “hot spots” characterized. You receive up to 15 different mutants that fail to interact. Use Loss of Affinity Mutants to obtain dominant negatives to speed up your functional studies and identify hotspots for small molecule screening! Have a look at these 2 publications for application examples: Journal of Biological Chemistry 2005 and Journal of Biological Chemistry 2006, or contact us for more information. |
