| Highly complex libraries |
don’t miss interactions !Our libraries are the most complex ones available on the market and ULTImate Y2H™ cell-to-cell mating is the only screening technology that can screen them exhaustively. This e-mail address is being protected from spam bots, you need JavaScript enabled to view it .Besides, our ULTImate Y2H libraries are validated by extremely stringent quality controls: - Verifying a complexity of at least 10 million independent, primary fragments in yeast for Random-Primed cDNA libraries. The complexity of the genomic libraries (constructed for organisms that contain no or few introns) is defined by the size of the genome (e.g. 2 million genomic fragments for Helicobacter pylori library). - Random sequencing of 192 clones to check i) absence of contamination, ii) that ribosomal RNA, mitochondrial DNA and empty vectors represent less than 10% of the total sequences. - Verifying the viability and mating properties of the library-containing yeast cells, and defining the conditions to obtain the highest mating efficiency (Patent US No 6,187,535) - Reproducing selected Y2H screen interactions available from the literature If you are interested in a specific library not currently listed, please contact us to discuss feasibility and conditions of custom-made libraries. |