You already know that your proteins interact. Now, further map the interacting domains on both partners and test discrete functional or structural features using 1-by-1 Y2H .

As little as a dozen of amino acids can be sufficient for an interaction. Use that information to derive interfering peptides or interaction-deficient variants for the functional validation of your interaction.

Mutually exclusive interactions or heterotrimeric complex? Map the interacting domains and get a hint!