You would like to test the interaction between candidate proteins? You would like to precisely map binding domains on known interacting proteins? You need to compare and quantify the binding between wild type and isoforms or mutants?
>> 1-by-1 Y2H offers you a simple, fast and robust answer. | |
Your proteins are cloned into our ULTImate Y2H™ plasmids and pairwise interactions are tested in yeast. Two reporter genes are used: His3 allows yeast cells to grow on a medium lacking histidine, and LacZ offers quantitative measurement of the interaction strength thanks to colorimetric or luminescent β-galactosidase substrates.
Please contact us for more information!
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